Week of 3/25-3/29: D. Metalli Biochemical Tests

Introduction

    This week we conducted biochemical tests on the bacteria D. Metalli in preparation for our conference presentation. These tests included the oxidase, urease, catalase, starch and SIM tests. These tests were conducted in order to better characterize the species. 

Methods

    In the beginning of the week we gram stained D. Metalli. Our results showed it to be a Gram positive rod shaped bacteria. We then streaked our experimental plates, including R2A and TGY plates for catalase and oxidase tests, a Starch TGY plate for Starch hydrolysis tests, and 2 experimental Urease plates and SIM Test tubes for D. Metalli. In addition we also streaked control plates/tubes with B. Subtilus, S. Epidermis, E. Coli, and C. Fruendii. The controls were incubated at 37℃ and the experimental groups at 30℃. The tests were then conducted on Wednesday. For the oxidase test, 2-3 drops of oxidase was put onto the plate. For the catalase test, drops of hydrogen peroxide were added to the plate. For the starch test, drops of IKI were put onto the plate. And Kovac's reagent was added to the tubes for the indole test.

Results

Gram Stain of D. Metalli

                                                                  
   From these tests, it was determined that D. metalli is positive for the oxidase, catalase, and starch tests, and negative for the urease, sulfur, indole, and motility tests.

Conclusions    

    The tests conducted provided further understanding of the bacteria D. metalli and provided us the information needed to continue on with presentation preparation.

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