Week of 9/16-9/20

This week, we poured 10 R2A plates and noted that the transformations performed on 9-12 and inoculated on 9-13 were unsuccessful. We tested Kanamycin’s efficiency on normal DRAD while also inoculating the most recent competent cells onto TGY with Kanamycin. To prepare for upcoming experiments, we planned additional plasmid extractions and made more media, including R2B and other types. We attempted to plate the previous transformation onto a TGY, Chloramphenicol, and Kanamycin plate and passaged 4-way E. coli into fresh LB ampicillin broth.

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