Week of 10/7-10/11

This week, we poured 10 TGY plates with 8 µg/mL Kanamycin and inoculated 3-way D. rad. into 25 mL of TGY with 8 µg/mL Kanamycin broth. We performed three transformations using previous plasmid extractions, but unfortunately, the transformations did not succeed. To check for contamination or other issues, we Gram-stained 3-way D. rad. from older broth stored in the fridge. Additionally, we rehydrated desiccated D. rad. cells using a rehydration buffer. We also passaged 4-way E. coli into fresh broth and inoculated more normal D. rad. in preparation for the NASA project. Finally, we performed three additional plasmid extractions on 4-way E. coli. The nanodrop values for these extractions are included via image.

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