Week of 11/4-11/8

This week, we inoculated 3-way D. rad. from a plate into TGY broth with 8 µg/mL Kanamycin but continued to encounter difficulty with its growth in the broth. We passaged 4-way E. coli into a new LB ampicillin broth, but a Gram stain revealed signs of contamination. To address this, we inoculated new LB ampicillin broth with 4-way E. coli from a freezeback and prepared 250 mL of LB Agar. A Gram stain of the new 4-way E. coli growth confirmed it was free of contamination. For the NASA project, desiccated D. rad. was unable to grow, so we inoculated normal D. rad. into TGY broth and R2B for desiccation. A Gram stain of this D. rad. confirmed it was also free of contamination.

Comments

Post a Comment

Popular posts from this blog

Week of 10/28-11/1

This week, we inoculated 4-way E. coli into LB broth with 50 µg/mL ampicillin and performed a Gram stain on the growth, confirming it was free of contamination. The growth was then passaged into another LB broth with 50 µg/mL ampicillin for further experimentation. We also redid the NASA project run by inoculating D. rad. onto R2B and TGY broths. Additionally, we performed four plasmid extractions, but the nanodrop readings from these extractions were not favorable. Due to the less-than-ideal nanodrop values, these plasmid extractions were ultimately not used.
Read more

Week of 3/25-3/29: D. Metalli Biochemical Tests

Image
Introduction      This week we conducted biochemical tests on the bacteria D. Metalli in preparation for our conference presentation. These tests included the oxidase, urease, catalase, starch and SIM tests. These tests were conducted in order to better characterize the species.  Methods      In the beginning of the week we gram stained D. Metalli. Our results showed it to be a Gram positive rod shaped bacteria.  We then streaked our experimental plates, including R2A and TGY plates for catalase and oxidase tests, a Starch TGY plate for Starch hydrolysis tests, and 2 experimental Urease plates and SIM Test tubes for D. Metalli. In addition we also s treaked control plates/tubes with B. Subtilus, S. Epidermis, E. Coli, and C. Fruendii. The controls were incubated at 37℃ and the experimental groups at 30 ℃. The tests were then conducted on Wednesday. For the oxidase test, 2-3 drops of oxidase was put onto the plate. For the catalase test, drops of h...
Read more

Week of 9/16-9/20

This week, we poured 10 R2A plates and noted that the transformations performed on 9-12 and inoculated on 9-13 were unsuccessful. We tested Kanamycin’s efficiency on normal DRAD while also inoculating the most recent competent cells onto TGY with Kanamycin. To prepare for upcoming experiments, we planned additional plasmid extractions and made more media, including R2B and other types. We attempted to plate the previous transformation onto a TGY, Chloramphenicol, and Kanamycin plate and passaged 4-way E. coli into fresh LB ampicillin broth.
Read more