Week of 11/11-11/15

This week, we rehydrated D. rad. growth for the NASA project and plated it onto respective plates for desiccation. A Gram stain of 3-way D. rad. from a plate confirmed it was free of contamination, and this culture was inoculated into a new TGY Kanamycin broth. We also passaged 4-way E. coli into fresh broth and performed three plasmid extractions from the culture. Successful growth of 3-way D. rad. in broth was verified by Gram stain, and a Gram stain of 4-way E. coli confirmed its growth in broth was also free of contamination. On 11/12, we conducted a gel on the plasmid extractions, which showed strong bands around 10,000 base pairs, as expected. Three transformations were performed, though contamination was found on one of the transformation plates. To address this, we re-inoculated the transformations and exposed them to 200 microjoules of UV for five minutes. Additionally, we conducted DNA extraction on 3-way D. rad. four times, with three of the trials yielding very favorable results. We plan to use this successful DNA extraction to perform DNA sequencing on 3-way D. rad.. Below are images of our DNA extraction values (1st image) and the gel run of our plasmid (2nd image).

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