Week of 4/1-4/5

 Introduction

    With the biochemical tests already conducted on D. Metalli, our focus on the bacteria was then shifted to its ability to form biofilm. Our goal was to bring about an even further understanding of the bacteria as well as provide potential follow up research on its biofilm in the future.

Methods

    Due to D. Metalli's growth of biofilm being more plentiful in nutrient rich environments, we inoculated the bacteria into 25 ml of TGY within a flask. This flask was then put inside of a non shaking incubator for 24 hours to best aid biofilm growth. 

Results    

    After the 24 hours, there was the successful growth of biofilm, as shown below:


In addition, we also gram stained D. Metalli with its biofilm growth, as shown below:


Conclusions

    The successful growth and observation of D. Metalli's biofilm proved to be very valuable information. We used this information for our conference poster and presentation. Further research as to how D. Metalli utilizes this biofilm can be potentially conducted in future projects as well.


Comments

Post a Comment

Popular posts from this blog

Week of 10/28-11/1

This week, we inoculated 4-way E. coli into LB broth with 50 µg/mL ampicillin and performed a Gram stain on the growth, confirming it was free of contamination. The growth was then passaged into another LB broth with 50 µg/mL ampicillin for further experimentation. We also redid the NASA project run by inoculating D. rad. onto R2B and TGY broths. Additionally, we performed four plasmid extractions, but the nanodrop readings from these extractions were not favorable. Due to the less-than-ideal nanodrop values, these plasmid extractions were ultimately not used.
Read more

Week of 3/25-3/29: D. Metalli Biochemical Tests

Image
Introduction      This week we conducted biochemical tests on the bacteria D. Metalli in preparation for our conference presentation. These tests included the oxidase, urease, catalase, starch and SIM tests. These tests were conducted in order to better characterize the species.  Methods      In the beginning of the week we gram stained D. Metalli. Our results showed it to be a Gram positive rod shaped bacteria.  We then streaked our experimental plates, including R2A and TGY plates for catalase and oxidase tests, a Starch TGY plate for Starch hydrolysis tests, and 2 experimental Urease plates and SIM Test tubes for D. Metalli. In addition we also s treaked control plates/tubes with B. Subtilus, S. Epidermis, E. Coli, and C. Fruendii. The controls were incubated at 37℃ and the experimental groups at 30 ℃. The tests were then conducted on Wednesday. For the oxidase test, 2-3 drops of oxidase was put onto the plate. For the catalase test, drops of h...
Read more

Week of 9/16-9/20

This week, we poured 10 R2A plates and noted that the transformations performed on 9-12 and inoculated on 9-13 were unsuccessful. We tested Kanamycin’s efficiency on normal DRAD while also inoculating the most recent competent cells onto TGY with Kanamycin. To prepare for upcoming experiments, we planned additional plasmid extractions and made more media, including R2B and other types. We attempted to plate the previous transformation onto a TGY, Chloramphenicol, and Kanamycin plate and passaged 4-way E. coli into fresh LB ampicillin broth.
Read more