Week of 10/14-10/18

This week, we conducted two Gram stains on potential 4-way D. rad. growth observed on a Chloramphenicol plate and passaged this growth onto a new Chloramphenicol plate. We prepared 250 mL of TGY Agar and poured 4 TGY Chloramphenicol plates with a concentration of 3 µg/mL and 5 regular TGY Agar plates. A Gram stain was performed on the new Chloramphenicol plate with potential 4-way D. rad. growth, and the growth was passaged again onto another new Chloramphenicol plate. However, a subsequent Gram stain on this most recent passage revealed that the transformation was unsuccessful and contaminated. We planned to perform new transformations and are considering exposing plates to UV light immediately before incubation to improve results.

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