Week of 9/2-9/6

The beginning of our week, Tuesday, saw us running a gel on our plasmid extractions from the prior week. The gel showed our plasmid to be the correct size, around 9000bp. The samples looked slightly dirty however, but still far better than the prior ones. We then conducted three plasmid extractions using our plasmids. These transformations, unfortunately, were again unsuccessful. In addition to being unsuccessful, our plated transformation plates showed contamination. We again gram stained our competent cells to esnsure this was not the source of contamination. These gram stains again showed no contamination. We were able to get successful growth of our 3-way D. rad by first inoculating from freezebac onto normal TGY plates free of antibiotic, then passaging that growth onto our typical 8µg/ml kanamycin plates. From the antibiotic plates, we then were able to get successful growth in antibiotic broth. The end of the week saw our group making new media for preparation of the following week.

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