Week of 9/23-9/27

This week, we performed three transformations: one using plasmid extraction A2, one with B2, and a control transformation with tube C2. We poured 20 plates in total, including 5 with 3 µg/mL Chloramphenicol TGY, 5 with 8 µg/mL Kanamycin TGY, and 10 with normal TGY. We passaged 3-way D. rad. into fresh TGY broth and performed DNA sequencing on the 4-way E. coli plasmid extraction, with results expected the following Monday. Additionally, we planned to carry out genomic DNA extraction on 3-way D. rad. in preparation for future experiments.

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