Week of 9/9-9/13

Having seen no success with our failed transformations, our group decided to begin considering a second project to work on simultaneously with our mutagenesis project. This project's goal is to test the limits of D. radiodurans' ability to repair its own DNA after extreme desiccation and radiation exposure. In addition to project planning, we inoculated the 3-way culture onto 8 µg/mL Kanamycin TGY broth and prepared 200 mL of TGY agar. We inoculated competent cells and the 3-way culture onto fresh plates and verified nanodrop readings for both. Additionally, we prepared new competent cells and performed three transformations using the most recent plasmid extractions.

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